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Bethesda creation kit fallout 4 console2/25/2023 ![]() ![]() I have done this below and called the column ‘ Absorbance (562 nm)‘: This will give background-corrected absorbance values. To do this, simply subtract the average absorbance values from the average absorbance value of the blank – so ‘ 0.0945‘. In this instance, before we can proceed we need to first correct the data for the background noise. ![]() What we have here is the average absorbances of each standard next to the corresponding known concentrations. To create the standard curve, I have measured the absorbance of 8 standards (25, 125, 250, 500, 750, 1000, 15 μg/mL total protein) and a blank sample (0 μg/mL total protein) for background corrections. The independent data is plotted on the x-axis, whereas the dependent data is plotted on the y-axis, on a scatter plot.įor this example, I will use data generated from the BCA protein assay kit to estimate total protein concentrations. the optical density readings of the samples). protein standard concentrations in a BCA assay), and the other is the dependent variable which refers to the measured values (e.g. ![]() One set of data must be the independent variable, which is the known values (e.g. To create a standard curve in Microsoft Excel, two data variables are required. I will use the BCA total protein assay standards as an example. In this guide I will explain how to create a linear standard curve using Microsoft Excel and how to use it to calculate unknown sample values. ![]()
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